Synergistic Effect of One Annonaceae and Two Rutaceae on The Stability of HbSS Red Blood Cells
Akakpo-Akue J1, Kplé Epouse Coulibaly TKM1*, Kra AKM1, Konan GKNA1, Fofié YNB2, Essy KF3, Sanogo I4, Yapo-Crezoit CCA5 and Djaman AJ1,5
Abstract
One of the consequences of the sickle cell is chronic hemolysis. The aim of this study was to determine the membrane-stabilizing potential of extracts of three plants: Zanthoxylum leprieurii, Xylopia aethiopica, and Harungara madagascariensis. The 70% hydroethanolic extract (EZHm) and the decocted (DZHm) were used to evaluate the protective effect of both extracts against the membranes HbSS hemolysis in the presence of increasing concentrations of NaCl respectively. The spectrophotometric method was employed in determining the osmotic fragility index of HbSS erythrocytes. From these results, it appears that the test extracts have the capacity to stabilize the membrane of HbSS erythrocytes. In the presence of the tested substances at the concentration of 0.625 mg/mL, the IC50 was 4, 3.23, and 3.9 for Phenylalanine, DZHm, and EZHm respectively, while at the concentration of 1.25 mg/mL, the IC50 was 3.30 2.43 and 2.39 for the same substances. There was a significant difference (p<0.01) between the NaCl-induced hemolysis inhibitory activity of DZHm and EZHm at 0.625 mg/mL compared to Phenylalanine at 0.625 mg/mL. The present study showed that the extracts from the combination of the three plants exhibited membrane stability potential on sickle cell erythrocytes.
Keywords
Antihemolytic activity; Zanthoxylum leprieurii; Xylopia aethiopica; Harungara madagascariensis.
Cite This Article
Akakpo-Akue, J., Kplé, E. C. TKM., Kra, AKM., Konan, GKNA., Fofié, YNB., Essy, KF., Sanogo, I., Yapo-Crezoit, CCA., Djaman, AJ. (2025). Synergistic Effect of One Annonaceae and Two Rutaceae on The Stability of HbSS Red Blood Cells. International Journal of Scientific Advances (IJSCIA), Volume 6| Issue 1: Jan-Feb 2025, Pages 179-183 URL: https://www.ijscia.com/wp-content/uploads/2025/02/Volume6-Issue1-Jan-Feb-No.840-179-183.pdf
Volume 6 | Issue 1: Jan – Feb 2025
