Administration of Oral Gemitir Flower Extract (Tagetes erecta L) Increased The Number of Neovascularization, Fibroblasts Cell and Epithelization of Wound in Male Wistar Strains Rats (Rattus norvegicus)
Sylvia Carolina Hutabarat*, I Gusti Made Aman, and Ni Wayan Winarti
Abstract
Background: Herbal ingredients that can be used to reduce wound healing on the skin are the administration of bioactive compounds from gemitir flowers (Tagetes erecta L) used as an anti-inflammatory and antioxidant. This study prove that oral gemitir extract increases the number of neovascularization, fibroblast cell and epithelialization of wound healing in male Wistar strains rats. Method: This research is anexperimental research with a randomized posttest-only control group design method. The research subjects used 28 male Wistar rats (Rattus norvegicus), which were divided into 2 control groups and 2 treatment groups. The analytical method used the Shapiro-Wilk Test and Levene’s Test and then compared using the one-way ANOVA post hoc with least significant difference (LSD). Results: Neovascularization P0-4 and P0-14 have the same value 1.86±0.90,while in group P1-48.86±1.35and P1-14 decreased to 5.00±1.15. Number of P0-4 fibroblast cells: 8.29±3.25and P014:9.00±3.51cells/field of view, while PI-4:18.57 ± 4.89 and P1-14: 32.00 ± 7.39. Epithelialization results as described by epithelial thickness at P0-4: 28.08 ± 10.07 µm and P0-14:298.86±273.88µm, while at P1-4: 1096.71±133.93µm and P1-14: 1674.00± 331.55µm. The results of the one way ANOVA p<0.001. LSD group P0 (4) vs P0 (14) were not significantly different while P0 (4) vs P1 (4), P0 (4) vs P1 (14), P1 (4) vs P1 (14) all differed significantly with p<0.05. Conclusion: Oral administration of ethanol extract of gemitir flowers increased neovascularization in wound healing male Wistar rats on day 4 and increased the number of fibroblasts and thickness of epithelialization on days 4 and 14.
Keywords
gemitir flower (Tagetes erecta L); neovascularization; fibroblasts; epithelialization (thick epithelial epidermis); wounds
Cite This Article
Hutabarat, S. C., Aman, I. G. M., Winarti, N. W. (2023). Administration of Oral Gemitir Flower Extract (Tagetes erecta L) Increased The Number of Neovascularization, Fibroblasts Cell and Epithelization of Wound in Male Wistar Strains Rats (Rattus norvegicus). International Journal of Scientific Advances (IJSCIA), Volume 4| Issue 3: May-Jun 2023, Pages 384-393, URL: https://www.ijscia.com/wp-content/uploads/2023/05/Volume4-Issue3-May-Jun-No.443-384-393.pdf
Volume 4 | Issue 3: May-Jun 2023
