Effect of Combination of Purple Sweet Potato (Ipomoea batatas L.) Ethanolic Extract and Ramipril Administration on Myocardial C-Reactive Protein Expression and Aortic Intima-Media Thickness in Hypertensive Rat Model
Gede Surya Ambara*, Bagus Ari Pradnyana D.S, and I Nyoman Wiryawan
Abstract
Purposes: To determine the effect of giving a combination of purple sweet potato (Ipomoea batatas L.) ethanolic extract and ramipril in a rat model of hypertension. Methods: This is an experimental study with a post-test-only control group design and is a collaborative study of the effect of giving a combination of purple sweet potato (Ipomoea batatas L.) ethanolic extract and ramipril in a rat model of hypertension. Thirty male Wistar rats were given 2 mL/day of a 4% NaCl diet to induce hypertension. The rats were divided into three treatment groups, consists of NaCl 4% 2 mL/day + combination therapy with purple sweet potato (Ipomoea batatas L.) ethanolic extract 400 mg/kgBW/day and ramipril 1 mg/day (P1), NaCl 4% 2 mL/day + purple sweet potato (Ipomoea batatas L.) ethanolic extract 400 mg/kgBW/day (P2) and NaCl 4% 2 mL/day + ramipril 1 mg/day (P3). At week 5, the rat was executed to examine myocardial CRP expression and measure aortic IMT. All the data obtained were analyzed statistically. Results: There was a significant difference in myocardial CRP expression between the three treatment groups (p value<0.05). The combination of purple sweet potato (Ipomoea batatas L.) ethanolic extract 400 mg/kgBW/day + ramipril 1 mg/day (P1) showed a better effect on reducing myocardial CRP expression than administration of purple sweet potato (Ipomoea batatas L.) ethanolic extract 400 mg/kgBW/day (P2) (mean difference 216.44 ng/ml; 95% CI 133.56 – 299.32; p value<0.001) or Ramipril 1 mg/day (P3) (mean difference 144.44 ng/ml; 95% CI 29 .40 – 199.47; p value=0.010). Administration of Ramipril 1 mg/day (P3) showed a better effect on reducing myocardial CRP expression than the group given purple sweet potato (Ipomoea batatas L.) ethanolic extract 400 mg/kg/day (P2) (mean difference 102.00 ng/ml; CI 95% 19 .11 – 184.88; p value=0.018). There was a significant difference in aortic IMT between the three treatment groups (p value<0.05). The combination of purple sweet potato (Ipomoea batatas L.) ethanolic extract 400 mg/kgBW/day + ramipril 1 mg/day (P1) showed a better effect on reducing aortic IMT than administration of purple sweet potato (Ipomoea batatas L.) ethanolic extract 400 mg/kgBW/day (P2) (mean difference 10,16 μm; 95% CI 6,63 – 13,69; p value<0.001) or Ramipril 1 mg/day (P3) (mean difference 5,10 μm; 95% CI 1,40 – 8,70; p value=0.008). Administration of Ramipril 1 mg/day (P3) showed a better effect on reducing aortic IMT than the group given purple sweet potato (Ipomoea batatas L.) ethanolic extract 400 mg/kg/day (P2) (mean difference 5,05 μm; CI 95% 1,50 – 8,50; p value=0.007). Combination of purple sweet potato (Ipomoea batatas L.) ethanolic extract and ramipril showed a higher potential effect on reducing myocardial CRP expression and aortic IMT compared to single therapy. Conclusion: The combination of purple sweet potato (Ipomoea batatas L.) ethanolic extract 400 mg/kgBW/day + ramipril 1 mg/day can provide the best decrease in the expression of myocardial CRP and aortic IMT compared to the administration of purple sweet potato (Ipomoea batatas L.) ethanolic extract 400 mg/kg/day or ramipril 1 mg/day.
Keywords
combination of purple sweet potato ethanolic extract and ramipril; myocardial CRP expression; aortic IMT; hypertension
Cite This Article
Ambara, G. S., Bagus, A. P. D. S., Wiryawan, I. N. (2023). Effect of Combination of Purple Sweet Potato (Ipomoea batatas L.) Ethanolic Extract and Ramipril Administration on Myocardial C-Reactive Protein Expression and Aortic Intima-Media Thickness in Hypertensive Rat Model. International Journal of Scientific Advances (IJSCIA), Volume 4| Issue 4: Jul-Aug 2023, Pages 667-675, URL: https://www.ijscia.com/wp-content/uploads/2023/08/Volume4-Issue4-Jul-Aug-No.490-667-675.pdf
Volume 4 | Issue 4: Jul-Aug 2023
