Neurogenesis of Adult Circulating Blood Mononuclears in Vitro

Dababseh I.M., Lesniak Y.I., Oleksenko N.P.

Abstract

Cellular therapy of injuries of the nervous system with autologous cellular material of the patient requires the development of methods of neurodifferentiation of stem and progenitor cells of the adult organism. The use of autologous cells is the most acceptable from the point of view of biosafety and compatibility. In our work, we investigated the possibility of directed neurogenesis of circulating blood precursors adhered fraction, obtained from the patient with spinal cord injury. These cells were cultured for 2 weeks using neuroinducers (retinoic acid, BDNF, NGF). During the first 3 days of cultivation, the adherent fraction of cells consisted of round cells of small and medium size with a large nucleus and a narrow rim of the cytoplasm, which gradually stratified and formed an oval and granular shape. Subsequent cultivation showed that the cells acquire morphological characteristics of different types of cells of the nervous system. At the end of this period, the predominant cell population was Nest+. The patient’s own cells after stimulation of neurogenic differentiation in vitro were removed, washed from medium components and injected into the spinal canal. Intracisterial and paraventricular administration of this fraction to the patient significantly improved impaired function.

Keywords

circulating blood precursors; neuroinduction; retinoic acid; BDNF; NGF; Nest + cells

Cite This Article

Dababseh, I. M., Lesniak, Y. I., Oleksenko, N. P. (2022). Neurogenesis of Adult Circulating Blood Mononuclears in Vitro. International Journal of Scientific Advances (IJSCIA), Volume 3| Issue 3: May-Jun 2022, Pages 368-372, URL: https://www.ijscia.com/wp-content/uploads/2022/06/Volume3-Issue3-May-Jun-No.270-368-372.pdf

Volume 3 | Issue 3: May-Jun 2022