The Effect of Alpha Lipoic Acid (ALA) Supplementation on the in Vitro Maturation Media of Goat Oocyte on Tumor Alpha Necrosis Factor (TNF-α) Expression and Malondialdehyde (MDA) Levels
Akhmad Afifudin Al-Anshori1, Imam Mustofa2, Erma Safitri2, Sri Mulyati2, Rimayanti Rimayanti2, Epy Muhammad Luqman2, and Widjiati Widjiati2*
Abstract
This study aimed to determine the effect of Alpha Lipoic Acid (ALA) supplementation on in vitro maturation media of goat oocytes on Tumor Necrosis Factor Alpha (TNF-α) expression and Malondialdehyde (MDA) levels. This laboratory experimental study consisted of three treatment groups: control group (G0), 25 μmol/L ALA supplementation (G1), and 50 μmol/L ALA supplementation (G2). A total of 366 goats Cumulus Oocyte Complex (COCs) were collected, then selected into 216 COCs, and matured in vitro for 22 hours in a 5% CO2 incubator, 98% humidity, 38.50C temperature. After that, TNF-α expression was identified using Immunocytochemistry staining with the addition of TNF-α antibody and calculated using the Remmele Scale Index. Measurement of MDA levels using the ELISA method. The data obtained were analyzed using Kruskal-Wallis, One Way ANOVA, and Duncan’s in the SPSS 24 software program (p<0.05). The expression value of TNF-α G0 was 0.75±1.39, G1 was 5.56±3.05, and G2 was 2.00±1.80. MDA levels of G0 were 26.52 ±2.92, G1 was 46.44 ±4.87, and G2 was 30.41±5.67. TNF-α expression data and MDA levels G0 and G2 didn’t show a significant difference, while G0 and G2 compared to G1 showed a significant difference (p<0.05). ALA supplementation of 25 µmol/L increased TNF-α expression or MDA levels and 50 µmol/L on in vitro maturation media of goat oocytes could reduce TNF-α expression or MDA levels than 25 µmol/L.
Keywords
alpha lipoic acid; in vitro maturation; MDA; TNF-α; reproductive health
Cite This Article
Al-Anshori, A. A., Mustofa, I., Safitri, E., Mulyati, S., Rimayanti, R., Luqman, E. M., Widjiati, W. (2023). The Effect of Alpha Lipoic Acid (ALA) Supplementation on the in Vitro Maturation Media of Goat Oocyte on Tumor Alpha Necrosis Factor (TNF-α) Expression and Malondialdehyde (MDA) Levels. International Journal of Scientific Advances (IJSCIA), Volume 4| Issue 1: Jan-Feb 2023, Pages 41-46, URL: https://www.ijscia.com/wp-content/uploads/2023/01/Volume4-Issue1-Jan-Feb-No.389-41-46.pdf
Volume 4 | Issue 1: Jan-Feb 2023
