The Effect of Chayote (Sechium Edule) Extract Cream Can Reduce Matrix Metalloproteinase-2 (MMP-2) Levels and Increase Collagen Density in The Wound Healing Process of Male Wistar Rats (Rattus Novergicus) With Diabetes Mellitus
Tuti Hartati1, I Wayan Weta2, Ni Made Linawati3, I Wayan Putu Sutirta Yasa4, I Gusti Ayu Artini5,and Anak Agung Gde Putra Wiraguna6
Abstract
Introduction: The main complication in Diabetes Mellitus patients is delayed wound healing resulting in chronic wounds. Chayote is known as a vegetable, which has anti-inflammatory properties. The tannin and saponin compounds in chayote produce antibacterial agents. This study aims to prove the role of chayote extract cream on the wound healing process of diabetic rats through MMP-2 markers and collagen density. Methods: This study was an experimental post-test only control group design using male rats. Samples were induced hyperglycaemia with 46 mg of nicotinamide and 10 mg of streptozotocin one week before the experiment. The sample was divided into 2 groups: the placebo group (P0) and the treatment group (P1) with a total of 11 samples per group. The treatment group received cream of chayote extract 60% for 1 week. On the 8th day, both groups were taken by punch biopsy to assess collagen density, then examined for MMP-2 levels in the blood. Results: The average MMP-2 level in the control group was 4.97 ± 0.36 ng/ml, while the average MMP-2 in the treatment group was 4.59 ± 0.42 ng/ml. The average collagen level in the control group was 37.08 ± 12.92 %/ body surface, while the mean collagen in the treatment group was 54.21 ± 15.76 %/ body surface. Conclusion: Administration of ethanol extract of chayote (Sechium edule) significantly reduced matrix metalloproteinase-2 (MMP-2) levels and increased collagen density in the wound healing process of rats.
Keywords
chayote; MMP-2; collagen; diabetes mellitus
Cite This Article
Hartati, T., Weta, I. W., Linawati, N. M., Yasa, I. W. P. S., Artini, I. G. A., Wiraguna, A. A. G. P. (2023). The Effect of Chayote (Sechium Edule) Extract Cream Can Reduce Matrix Metalloproteinase-2 (MMP-2) Levels and Increase Collagen Density in The Wound Healing Process of Male Wistar Rats (Rattus Novergicus) With Diabetes Mellitus. International Journal of Scientific Advances (IJSCIA), Volume 4| Issue 4: Jul-Aug 2023, Pages 587-591, URL: https://www.ijscia.com/wp-content/uploads/2023/07/Volume4-Issue4-Jul-Aug-No.475-587-591.pdf
Volume 4 | Issue 4: Jul-Aug 2023
