The Effect of Ethanol Extract of Pearl Grass (Hedyotis corymbosa (L.) Lamk) on TNF-α and IL-10 Levels in Mice Infected with Plasmodium berghei

Dinda Eka Putri¹, Hartono Kahar^2,3, Heny Arwati^2,4

Abstract

Pearl grass (Hedyotis corymbose, (L.) Lamk) has been reported to have antimalarial activity against Plasmodium falciparum and Plasmodium berghei because of its chemical contents as well as anti-inflammatory activity. This current study aimed to analyse the effect of ethanolic extract of pearl grass (EEHC) on the serum level of TNF-α and IL-10 in mice infected with P. berghei. Fifty male BALB/c mice were infected with 1×10⁶ P. berghei-infected erythrocytes from donor mice, and then were randomly divided into five groups. Each group contained 10 mice. Group 1 was a positive control group, and treated with 187.2 mg/Kg BW antimalaria drug of dihydroartemisinin piperaquine (DHP). Group 2 was a negative control group without any additional drugs. Group 3, 4, and 5 were treated with 250, 300, and 350 mg/Kg BW of EEHC, respectively.  A curative test of EEHC was started on day three post-infection, followed by daily treatments of two hundred microliters of EEHC for four consecutive days. Parasitaemia was observed on day five post-treatment on Giemsa-stained tail blood smears followed by cardiac puncture to obtain sera prior to TNF-α and IL-10 measurement by ELISA.  The findings showed that higher doses of EEHC reduced parasitemia, which increases their therapeutic impact. Serum level of TNF-α was lower in mice treated with higher doses of EEHC, while IL-10 was vice versa. Serum level of TNF-α was correlated with percentage parasitemia, whereas IL-10 was not. These findings demonstrated EEHC’s anti-inflammatory effect against malaria in mice, suggesting that it may be developed as an antimalarial drug in the future.

Keywords

Ethanolic extract of Hedyotis corymbose; mouse malaria; anti-inflammatory; parasitemia.

Cite This Article

Putri, D. E., Kahar, H., Arwati, H. (2024). The Effect of Ethanol Extract of Pearl Grass (Hedyotis corymbosa (L.) Lamk) on TNF-α and IL-10 Levels in Mice Infected with Plasmodium berghei. International Journal of Scientific Advances (IJSCIA), Volume 5| Issue 4: Jul-Aug 2024, Pages 725-729, URL: https://www.ijscia.com/wp-content/uploads/2024/07/Volume5-Issue4-Jul-Aug-No.641-725-729.pdf

Volume 5 | Issue 4: Jul-Aug 2024