The Effect of Ethanol Extract of Pearl Grass (Hedyotis corymbosa (L.) Lamk) on TNF-α and IL-10 Levels in Mice Infected with Plasmodium berghei
Dinda Eka Putri1, Hartono Kahar2,3, Heny Arwati2,4
Abstract
Pearl grass (Hedyotis corymbose, (L.) Lamk) has been reported to have antimalarial activity against Plasmodium falciparum and Plasmodium berghei because of its chemical contents as well as anti-inflammatory activity. This current study aimed to analyse the effect of ethanolic extract of pearl grass (EEHC) on the serum level of TNF-α and IL-10 in mice infected with P. berghei. Fifty male BALB/c mice were infected with 1×106 P. berghei-infected erythrocytes from donor mice, and then were randomly divided into five groups. Each group contained 10 mice. Group 1 was a positive control group, and treated with 187.2 mg/Kg BW antimalaria drug of dihydroartemisinin piperaquine (DHP). Group 2 was a negative control group without any additional drugs. Group 3, 4, and 5 were treated with 250, 300, and 350 mg/Kg BW of EEHC, respectively. A curative test of EEHC was started on day three post-infection, followed by daily treatments of two hundred microliters of EEHC for four consecutive days. Parasitaemia was observed on day five post-treatment on Giemsa-stained tail blood smears followed by cardiac puncture to obtain sera prior to TNF-α and IL-10 measurement by ELISA. The findings showed that higher doses of EEHC reduced parasitemia, which increases their therapeutic impact. Serum level of TNF-α was lower in mice treated with higher doses of EEHC, while IL-10 was vice versa. Serum level of TNF-α was correlated with percentage parasitemia, whereas IL-10 was not. These findings demonstrated EEHC’s anti-inflammatory effect against malaria in mice, suggesting that it may be developed as an antimalarial drug in the future.
Keywords
Ethanolic extract of Hedyotis corymbose; mouse malaria; anti-inflammatory; parasitemia.
Cite This Article
Putri, D. E., Kahar, H., Arwati, H. (2024). The Effect of Ethanol Extract of Pearl Grass (Hedyotis corymbosa (L.) Lamk) on TNF-α and IL-10 Levels in Mice Infected with Plasmodium berghei. International Journal of Scientific Advances (IJSCIA), Volume 5| Issue 4: Jul-Aug 2024, Pages 725-729, URL: https://www.ijscia.com/wp-content/uploads/2024/07/Volume5-Issue4-Jul-Aug-No.641-725-729.pdf
Volume 5 | Issue 4: Jul-Aug 2024
