The Potential of Alcohol and Triclosan, Alcohol and Chloroxylenol, and the Antibiotic Azithromycin Combinations as Anti-Biofilm Agents Against Pseudomonas aeruginosa
Muhammad Daffa Alva Hernanda1, Syauqie Alifian Wandhara1, Tri Pudy Asmarawati2, Wiwin Is Effendi3, Agung Dwi Wahyu Widodo4*
Abstract
Background: Pseudomonas aeruginosa is a gram-negative bacterium commonly found in the environment and known as an opportunistic pathogen capable of forming biofilms. Biofilm formation makes the bacteria more resistant to conventional treatments, necessitating effective anti-biofilm agents. This study aims to evaluate the potential of combining alcohol and triclosan, alcohol and chloroxylenol, and the antibiotic azithromycin as anti-biofilm agents against Pseudomonas aeruginosa. Method: The research was conducted using experimental methods in the laboratory, utilizing clinical isolates of Pseudomonas aeruginosa from Dr. Soetomo General Hospital. The bacteria were incubated in media containing these combinations of antibiofilm agents, and their effects were measured based on the optical density of the formed biofilm. Result: The combinations of alcohol and triclosan, alcohol and chloroxylenol, and azithromycin showed significant effects in inhibiting the formation of Pseudomonas aeruginosa biofilms. The combination of alcohol and triclosan had the strongest antibiofilm effect compared to the other combinations. Conclusion: The combinations of alcohol and triclosan and alcohol and chloroxylenol are effective as anti-biofilm agents against Pseudomonas aeruginosa, with the combination of alcohol and triclosan showing the highest potential. In contrast, azithromycin exhibited comparatively lower efficacy in inhibiting biofilm formation relative to the other combinations. Further research is needed to test the effectiveness and safety of these combinations in clinical settings.
Keywords
pseudomonas aeruginosa; biofilm; antimicrobial resistance; anti-biofilm; antibacterial; antibiotic.
Cite This Article
Hernanda, M. D. A., Wandhra, S. A., Asmarawati, T. P., Effendi, W. I., Widodo, A. D. W. (2024). The Potential of Alcohol and Triclosan, Alcohol and Chloroxylenol, and the Antibiotic Azithromycin Combinations as Anti-Biofilm Agents Against Pseudomonas aeruginosa. International Journal of Scientific Advances (IJSCIA), Volume 5| Issue 6: Nov-Dec 2024, Pages 1405-1410, URL: https://www.ijscia.com/wp-content/uploads/2024/12/Volume5-Issue6-Nov-Dec-No.742-1405-1410.pdf
Volume 5 | Issue 6: Nov – Dec 2024
