Topical Administration of Red Gedi Leaves (Abelmoschus Manihot L. Medik) Gel Extract Increases Neovascularization, Number of Fibroblasts and Re-Epithelialization on Wounds in Diabetic Rats
Pingkan Maria Runtuwene1*, Ketut Kwartantaya Winaya2, and Ni Nyoman Ayu Dewi3
Abstract
Flavonoids (quercetin), saponins, tannins, alkaloids and phenols (gallic acid) in red gedi leaves (Abelmoschus manihot L. Medik) can accelerate wound healing which is often found in diabetes mellitus. This was an experimental randomized post-test only control group design using 30 male Wistar rats with diabetes mellitus that were divided into 6 groups. On the 4th and 14th days after treatment, skin tissue samples were taken, histology preparations were made using Hematoxilin-Eosin (HE) staining to examine neovascularization, number of fibroblasts and re-epithelialization. Comparative analysis showed that the average neovascularization on the 4th day of the treatment I group was higher than treatment group II and the lowest was in the control group. Meanwhile, on 14th day, treatment group I had the highest neovascularization rate (p<0.001). The average of fibroblasts in treatment group I on the 4th day higher than treatment group II and the lowest in the control group. Meanwhile, on 14th day, treatment group I had the highest fibroblast average (p<0.001). The mean re-epithelialization on 4th day was highest in treatment group I and lowest in the control group. Meanwhile, on the 14th day, treatment group I had the highest re-epithelialization average (p<0.001). In conclusion, administration of red gedi leaf ethanol gel extract with concentrations of 12.5% and 25% increased neovascularization, number of fibroblast cells and re-epithelialization in wound healing of male Wistar rats with diabetes mellitus, with a concentration of 12.5% being better for all parameters.
Keywords
anti-aging; red gedi leaf (Abelmoschus Manihot L. Medik); neovascularization; fibroblast, re-ephithelization
Cite This Article
Runtuwene, P. M., Winaya, K. K., Dewi, N. N. A. (2023). Topical Administration of Red Gedi Leaves (Abelmoschus Manihot L. Medik) Gel Extract Increases Neovascularization, Number of Fibroblasts and Re-Epithelialization on Wounds in Diabetic Rats. International Journal of Scientific Advances (IJSCIA), Volume 4| Issue 6: Nov-Dec 2023, Pages 998-1007, URL: https://www.ijscia.com/wp-content/uploads/2023/12/Volume4-Issue6-Nov-Dec-No.533-998-1007.pdf
Volume 4 | Issue 6: Nov-Dec 2023
